AAGP™ Protects Human Neonatal Skin Fibroblasts Against Low Temperature (-3°C and 3°C) Induced Cell Death

Husseing and Retamal, December 2005

Introduction

It is becoming increasingly apparent that AAGP™ has strong cell preservation qualities. With this in mind a series of experiments mimicking cell stress conditions (pH, oxidative stress, low temperatures and time) were conducted to challenge both human adult and neonatal fibroblasts exposed to AAGP™ in comparison to human adult and neonatal fibroblasts not exposed to AAGP™. This was done with the use of AAGP™ as a anti- aging cosmetic, protecting skin from the harsh environment, in mind. The first of these experiments is outlined below and aimed to elucidate the protective effects of AAGP™ at low temperatures (-3°C and 3°C)

Method

Neonatal skin fibroblasts cells were plated at a concentration of 2x105 cells/ml and grown on non-adherent plates in low serum media at temperatures of -3°C or 3°C. To test the affects of AAGP™ at different concentrations the cells were exposed to either 0, 10 or 15mg/ml AAGP™ throughout the course of the experiment. Cell counts were taken at 0, 24 and 34 hours using Trypan blue and two counts were performed per sample to calculate the percentage viability (from 0 hours) of the cell sample.

Results

The ability of AAGP™ to protect against temperature induced cell death can be seen as early as 1hour into the experiment. By 34 hours cells exposed to temperatures of -3 or 3°C were at only 20% viability when in the presence of no AAGP™. However, at both temperatures cells exposed to 10 or 15mg/ml AAGP™ maintained viability percentages of above 80% (96% for 10 and 15mg/ml AAGP™ exposed cells at -3°C).


Figure 1: Effects of AAGP™ on the survival of human neonatal fibroblasts at 3°C.


Figure 2: Effects of AAGP™ on the survival of human neonatal fibroblasts at -3°C.

Conclusion

These results conclude that AAGP™ is able to protect cells against low-temperature induced cell death for up to 34 hours. The mechanism by which this occurs is presently unknown.